Recently, a research team led by Professor Shuo Huang's team from the School of Chemistry at Nanjing University has achieved a major breakthrough in single-molecule peptide sequencing with a novel nanopore "chop and measure" method. By combining stepwise enzymatic cleavage with iterative N-terminus re-reading, this method enables precise identification of single-amino-acid mutations, post-translational modifications and unnatural amino acid insertions, demonstrating broad potential in proteomics and chiral peptide analysis. This work was published online on July 29, 2026, in the journal Nature under the title "Sequential reading of a stepwise-shortened peptide immobilized on nanopore".

Fig. Construction of a hetero-octameric MspA containing dual reactive sites.

Source: https://www.nature.com/articles/s41586-026-10881-1